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鳉胚胎和细胞移植嵌合体生成Dechorionation
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras
DOI:

09:03 min

December 22, 2010

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Chapters

  • 00:05Title
  • 01:02Introduction
  • 01:32Dechorionating Medaka Eggs
  • 04:22Cell Transplantation in Medaka Embryos
  • 06:05Mounting Dechorionated Embryos
  • 07:35Images of Post-Transplantation Embryos
  • 08:31Conclusion

Summary

자동 번역

由于硬的蛋壳和软的胚胎,操纵鳉胚胎是比在斑马鱼中更多地参与。该视频显示一步一步的程序,包括dechorionation如何操作鳉胚胎,在琼脂糖凝胶成像和细胞移植嵌合体的生产安装。这些程序是必不可少的,在实验室中使用鳉和斑马鱼为脊椎动物的基因组功能基因清扫充分利用其互补性的特点。

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