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Journal / Biology / 組織サンプルのγH2AX病巣の定量
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組織サンプルのγH2AX病巣の定量
 
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組織サンプルのγH2AX病巣の定量

Article DOI: 10.3791/2063-v • 08:48 min • June 28th, 2010
June 28th, 2010 •
1,2, 1,3, 1,2,3, 1, 2,3, 3,4,5, 1,3
1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 3Department of Pathology, The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children's Research Institute, Royal Children's Hospital, 5Department of Pediatrics, The University of Melbourne

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γH2AX巣に基づいてDNA二本鎖切断の定量は、組織の放射線感受性と放射線修正する化合物の作用の評価に、特に放射線の生物学では、非常に貴重なツールとなっています。ここでは、組織サンプルにおけるγH2AX病巣の定量のための免疫蛍光アッセイの使用方法を示しています。

Tags

細胞生物学、問題40、蛍光抗体法、DNA二本鎖切断、ヒストンバリアント、H2AX、DNA損傷、電離放射線、活性酸素種
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