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Chapters
- 00:09Collecting pre-pupae
- 00:36Dissecting and fixing wings
- 01:08Pre-hybridization
- 02:27Making hybridization buffer
- 03:09Hybridization
- 03:59Applying antibody
- 04:31Detection and development
- 05:35Mounting
- 05:59Result: patterns of Engrailed
In order to examine gene expression in the pupal wing tissue of Bicyclus anynana, we present an optimized protocol for in situ hybridizations using riboprobes. We also provide guidelines for the further optimization of this protocol for use in pupal wings of other Lepidopteran species.
