Journal
/
/
Isolatie, Verrijking, en onderhoud van Medulloblastoom Stem Cells
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
DOI:

06:32 min

September 01, 2010

, , ,

Chapters

  • 00:00Title
  • 00:59Introduction
  • 01:20Micro-dissection of Tumor-bearing Cerebellum and Dissociation of Tumor Tissue
  • 03:34Enrichment, Maintenance, and Expansion of Medulloblastoma Tumor Cells by Serial Passages
  • 04:26Immunofluorescent Staining of Cultured Medulloblastoma Cells
  • 04:53Morphology, Lineage Differentiation, and Expression of Stem Cells Markers
  • 06:17Conclusion

Summary

자동 번역

Dit protocol beschrijft de isolatie, verrijking en het onderhoud van medulloblastoom tumor stamcellen uit mutante muizen met ectopische Sonic Hedgehog pathway activiteit.

Related Videos

Read Article