Journal
/
생물학
/
An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase

DOI:
10.3791/2094-v

•

08:08 min

•

December 19, 2010

•

,
1Department of Biochemistry,University of Toronto

Chapters

  • 00:05Title
  • 01:10Introduction
  • 01:39Enzyme Assay Preparation
  • 02:51Lysine Decarboxylase (Ldcl) Enzyme Assay
  • 04:17Trinitrobenzene Sulfonic Acid (TNBS) Reaction and Color Development
  • 06:42Ldcl Assay Representative Results
  • 07:31Conclusion

Summary

자동 번역

자동 번역

The activity of the inducible lysine decarboxylase is monitored by reacting the substrate L-lysine and the product cadaverine with 2,4,6-trinitrobenzensulfonic acid to form adducts that have differential solubility in toluene.

Tags

AssayMeasuringActivityEscherichia ColiInducible Lysine DecarboxyasePHAcidic PH SurvivalAmino Acid DecarboxylasesGlutamic Acid DecarboxylasesArginine DecarboxylaseLysine DecarboxylaseOrnithine DecarboxylasePyridoxal-5′-phosphateCo-factorInner-membrane Substrate-product AntiportersLysine-cadaverine AntiporterCadBX-ray Crystal StructureStringent Response Regulator Guanosine 5′-diphosphate 3′-diphosphatePpGppStringent Response

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/2094?article_type=v

Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap
check_url/kr/2094?article_type=v

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
check_url/kr/2094?article_type=v

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
check_url/kr/2094?article_type=v

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
check_url/kr/2094?article_type=v

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
check_url/kr/2094?article_type=v

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
check_url/kr/2094?article_type=v

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
check_url/kr/2094?article_type=v

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
check_url/kr/2094?article_type=v

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
check_url/kr/2094?article_type=v

Measuring Lactase Enzymatic Activity in the Teaching Lab

Read Article