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用于测量的活性测定大肠埃希氏菌诱导赖氨酸Decarboxyase
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An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
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用于测量的活性测定大肠埃希氏菌诱导赖氨酸Decarboxyase

DOI:
10.3791/2094-v

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08:08 min

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December 19, 2010

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,
1Department of Biochemistry,University of Toronto

Chapters

  • 00:05Title
  • 01:10Introduction
  • 01:39Enzyme Assay Preparation
  • 02:51Lysine Decarboxylase (Ldcl) Enzyme Assay
  • 04:17Trinitrobenzene Sulfonic Acid (TNBS) Reaction and Color Development
  • 06:42Ldcl Assay Representative Results
  • 07:31Conclusion

Summary

자동 번역

자동 번역

2,4,6 - trinitrobenzensulfonic酸反应的底物L -赖氨酸和产品尸形成加合物在甲苯的溶解度差，诱导赖氨酸脱羧酶的活动都受到监控。

Tags

AssayMeasuringActivityEscherichia ColiInducible Lysine DecarboxyasePHAcidic PH SurvivalAmino Acid DecarboxylasesGlutamic Acid DecarboxylasesArginine DecarboxylaseLysine DecarboxylaseOrnithine DecarboxylasePyridoxal-5′-phosphateCo-factorInner-membrane Substrate-product AntiportersLysine-cadaverine AntiporterCadBX-ray Crystal StructureStringent Response Regulator Guanosine 5′-diphosphate 3′-diphosphatePpGppStringent Response

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