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双荧光原位杂交食脑切片

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Double Fluorescence in situ Hybridization in Fresh Brain Sections

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双荧光原位杂交食脑切片

DOI:

•

12:15 min

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August 14, 2010

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Jin Kwon Jeong, Zhuoxun Chen, Liisa A. Tremere, Raphael Pinaud²

¹Department of Brain and Cognitive Sciences,University of Rochester, ²Center for Visual Science,University of Rochester

Chapters

00:04Title
00:07Introduction
00:56Prep of Sephadex G-50 Columns
02:54Labeling and Purification of Riboprobes
04:25Post-fixation, Acetylation, and Hybridization
07:22Detection and Visualization of Riboprobes
10:35Representative Results
11:35Conclusion

Summary

자동 번역

该协议涉及非放射性<em>原位</em>杂交程序，使在脊椎动物的大脑薄片，同时识别两个种成绩单，在一个单细胞的决议。

Tags

Double Fluorescence In Situ Hybridization DFISH MRNA Fresh Frozen Brain Sections Gene Co-regulation Anatomical Organization Neurochemical Properties Sensory Experience Central Sensory Circuits Single Cell Resolution Protocol Validation Mice Rats Songbirds Vertebrate Species

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