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RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells
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Chapters
- 00:00Title
- 00:58Introduction
- 01:19RNA Interference in 384-well Plates
- 03:09Infecting with Vaccinia Virus
- 04:11Staining Plates
- 06:02Representative Images and Interpretation of Infection Rates
- 07:35Conclusion
Novel host factors involved in viral infection can be identified through cell-based genome-wide loss of function RNAi screening. A Drosophila cell culture model is particularly amenable to this approach due to the ease and efficiency of RNAi. Here we demonstrate this technique using vaccinia virus as an example.
Tags
RNAi ScreeningHost FactorsVaccinia Virus InfectionDrosophila CellsViral PathogensPublic Health ThreatBioterrorismTherapeutics DevelopmentRNA InterferenceGenome-wide ScreensLoss-of-function ScreensMammalian VirusesMedical ImportanceAgricultural ImportanceVirus Infection StepsEntryTranslationRNA ReplicationCellular FactorsViral ReplicationHuman Cells Infected With VirusesAntiviral Therapeutics Development
