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Chapters
- 00:00Title
- 00:58Introduction
- 01:56Preparation of Neurons
- 03:38Click-iT 5-ethynyl-2-deoxyuridine (EdU) Labeling
- 05:31Tyramide Signal Amplification (TSA) of EdU Signal
- 07:09Visualization of Mitochondrial DNA Replication as a Marker for Mitochondiral Biogenesis
- 08:37Conclusion
Vi utvecklade en känslig teknik för att märka nyligen syntetiserade mitokondrie-DNA (mtDNA) i enskilda celler för att studera mtDNA biogenes. Tekniken kombinerar införlivandet av EDU tillsammans med en tyramide signalförstärkning (TSA) protokoll för att visualisera mtDNA replikation inom subcellulära fack av nervceller.
Tags
VisualizationMitochondrial DNA ReplicationCellsEdU Signal AmplificationMitochondriaCellular EnergyMitochondrial BiogenesisMtDNA Replication RateSensitive TechniqueLabelingNewly Synthesized MtDNAIndividual CellsMtDNA Biogenesis Study5-ethynyl-2′-deoxyuridine (EdU)Tyramide Signal Amplification (TSA)Visualization Of MtDNA ReplicationSubcellular CompartmentsNeuronsThymidine Analogs5-bromo-2-deoxyuridine (BrdU)Click ReactionAcid TreatmentsEnzyme DigestsEdU Incorporation ComparisonCellular MarkersMechanisms Of Mitochondrial Biogenesis RegulationPathogenesis Associated With Drug ToxicityAgingCancerNeurodegenerative Diseases
