Journal
/
생물학
/
Injection of dsRNA into Female A. aegypti Mosquitos
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Injection of dsRNA into Female A. aegypti Mosquitos

Injection of dsRNA into Female A. aegypti Mosquitos

DOI:
10.3791/215-v

•

04:24 min

•

July 04, 2007

•

, ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics,University of California, Irvine (UCI)

Chapters

  • 00:07Introduction
  • 00:37Preparation of Needles for Injection of dsRNA
  • 01:35The Injection Setup
  • 02:47Injection of dsRNA
  • 03:55Conclusion
  • 04:17End Credits

Summary

자동 번역

자동 번역

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Tags

DsRNA InjectionReverse Genetic ApproachA. Aegypti MosquitoesVector PathogensJames LabDengue VirusCalibrating Injection NeedlesManipulating Injection SetupInjecting DsRNAThorax

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes

Intraperitoneal Injection into Adult Zebrafish

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development

RNAi Interference by dsRNA Injection into Drosophila Embryos

RNAi-medieret Gene Knockdown og<em> In Vivo</em> Diurese Assay i Voksen Kvinde<em> Aedes aegypti</em> Myg

Måling peptid translokation i store Unilamellar Vesikler

Fysiologiske Recordings og RNA-sekventering af smags- Vedhæng i gul-feber Mosquito<em> Aedes aegypti</em

RNAi Trigger Levering ind<em> Malariamyg</em> Pupper

Read Article