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RNAを含むオリゴヌクレオチドを​​使用して変換によるゲノムDNAのRNA / DNAハイブリッドの世代
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
DOI:

16:42 min

November 24, 2010

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Chapters

  • 00:05Title
  • 01:20Introduction
  • 01:53Preparation of the RNA-containing Oligo and the Yeast Cells for Transformation
  • 04:40Transformation of Yeast Cells Using the RNA-Containing Oligo
  • 07:47Analysis of Gene Correction by the RNA-Containing Oligo
  • 11:10Alkali Treatment of the RNA-Containing Oligo
  • 12:20Representative Results for Transformation Using RNA-Containing Oligonucleotides
  • 16:10Conclusion

Summary

자동 번역

この作品は、染色体レベルでのRNA / DNAハイブリッドを形成し、酵母細胞内でRNAからゲノムDNAに遺伝情報の伝達を明らかにする方法を示しています。

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