JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:13Introduction
- 01:42Isolation of Heart Valve Leaflets
- 03:03Isolation of Endothelial Layer
- 05:22Isolation of Side Specific Endothelial Layer
- 08:03Isolation of Interstitial Cells
- 09:07Isolation of Valvular Endothelial Cells Representative Images
- 10:30Conclusion
Tags
IsolationValvular Endothelial CellsHeart ValvesUnidirectional Blood FlowCardiovascular SystemMechanical StressesValvular Endothelial CellsInterstitial CellsRepair And RemodelIn Vitro ExperimentationIsolation And CultureDirect IsolationSide Specific IsolationCultureVerification Of Pure PopulationsEnzymatic DigestionGentle Swab Scraping TechniqueSurface CellsCentrifuged Into A PelletResuspended And PlatedCollagen I MatrixVEC PhenotypeContact Inhibited GrowthEndothelial Specific Markers (PECAM1/CD31)Von Willebrand Factor (vWF)Alpha-smooth Muscle Actin (α-SMA)Functional Characteristics
