JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
A Technique to Develop a Parasite Infection in Intestinal Organoids via Microinjection
내레이션 대본
To microinject the parasites into the apical side of a 3D organoid, first, use a micropipette puller to prepare glass injection capillaries. Use forceps to cut the tip of the capillary to a 9- to 12-micrometer diameter, to enable an easy flow of sporozoites, or oocysts if you choose to inject oocysts directly, and use micro-loader tips to fill each capillary with a fast green dye-labeled oocyst or sporozoite suspension.
Then, load the first sporozoite-filled capillary into a microinjector, and use an inverted microscope at a 5x magnification and a constant pressure to microinject 100 to 200 nanoliters of the suspension into each organoid.
