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JoVE Journal
Immunology and Infection

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Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
 
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Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells

Article DOI: 10.3791/2186-v 11:22 min October 6th, 2010
October 6th, 2010

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This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.

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Co-culture Models Pseudomonas Aeruginosa Biofilms Human Airway Cells CFBE Cells Static Co-culture Biofilm Model Flow Cell Co-culture Biofilm Model Arginine Bacterial Biofilms Epithelial Cells Biofilm Development Tissue Culture Plates Glass Coverslip Peristaltic Pump Fresh Medium Visualization Green Fluorescent Protein (GFP) Cystic Fibrosis (CF) Lung
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