JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Introduction
- 00:29Embryo Collection
- 02:26Preparing Microinjection Needles
- 05:30Alignment and Manipulation of Embryos
- 08:22Dessication of Embryos
- 10:18Micromanipulation and Microinjection
- 13:59Post Injection Treatment of Embryos
- 15:25Hatching of Larvae
- 16:50Conclusion
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, dessicating embryos, and performing microinjection are demonstrated.
