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Chapters
- 00:05Title
- 01:48Introduction
- 02:51Creation and Preparation of Peptides and Lytic Peptide Conjugates
- 03:43Extraction of Termite Gut Protozoa Under Anaerobic Conditions
- 06:59Binding of Fluorescent-coupled Ligand to Termite Gut Protozoa
- 08:12Binding Ligand-coupled Lytic Peptides to Termite Gut Protozoa
- 09:07Injecting Fluorescent-coupled Ligand into the Termite Hindgut
- 12:08Injecting Ligand-coupled Lytic Peptide into the Termite Hindgut
- 12:59Results: Synthesized Ligand Increases Lytic Peptide Binding and Activity Against Termite Gut Protozoa
- 15:14Conclusion
We present procedures for demonstrating that ligands bind to the surface membrane of the cellulose-digesting protozoa in the gut of Formosan subterranean termites using fluorescent microscopy and that ligands coupled with lytic peptides kill these protozoa in vitro (anaerobic protozoa culture) and in vivo (injection into the termite hindgut).
Tags
Protozoacidal ActivityLigand-lytic PeptidesTermite Gut ProtozoaIn VitroIn VivoSubterranean Termite ControlChemical PesticidesHindgutLytic PeptidesProtozoan ParasitesFormosan Subterranean TermiteNonspecific Immune SystemMicroorganismsHigher EukaryotesCell MembranesLigandCancer Cell Membrane Receptor LigandsBreast Cancer CellsNon-specific PeptidesHuman HormonesTargeted Destruction
