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Provning Protozoacidal aktivitet Ligand-lytisk Peptider Mot Termite Protozoa Gut In vitro (Protozoa Kultur) och In vivo (Mikroinjektion i Termite hindgut)
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JoVE 신문 생물학
Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)
DOI:

17:36 min

December 29, 2010

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Chapters

  • 00:05Title
  • 01:48Introduction
  • 02:51Creation and Preparation of Peptides and Lytic Peptide Conjugates
  • 03:43Extraction of Termite Gut Protozoa Under Anaerobic Conditions
  • 06:59Binding of Fluorescent-coupled Ligand to Termite Gut Protozoa
  • 08:12Binding Ligand-coupled Lytic Peptides to Termite Gut Protozoa
  • 09:07Injecting Fluorescent-coupled Ligand into the Termite Hindgut
  • 12:08Injecting Ligand-coupled Lytic Peptide into the Termite Hindgut
  • 12:59Results: Synthesized Ligand Increases Lytic Peptide Binding and Activity Against Termite Gut Protozoa
  • 15:14Conclusion

Summary

자동 번역

Vi presenterar förfaranden för att påvisa att ligander binder till ytan membranet i protozoer cellulosa-smälta protozoer i tarmen av Formosan underjordiska termiter med fluorescerande mikroskopi och att ligander tillsammans med lytisk peptider dödar dessa<em> In vitro</em> (Anaerob protozoer kultur) och<em> In vivo</em> (Injektion i termit hindgut).

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