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1Department of Basic Science and Craniofacial Biology,College of Dentistry, New York University, 2Department of Internal Medicine and Department of Psychiatry,School of Medicine, Washington University in St. Louis, 3Veterans Affairs Medical Center, 4School of Dental Medicine, Department of Biochemistry,University of Pennsylvania-School of Medicine, 5Monell Chemical Senses Center, 6Monell Chemical Senses Center
Chapters
- 00:00Title
- 00:11Introduction
- 01:54Subject Preparation
- 03:39Tongue Papillae Removal Technique
- 06:54Representative Papillae Tissue Results
- 08:55Conclusion
La connaissance des mécanismes moléculaires sous-jacents de transduction gustative a récemment connu des avancées significatives, en grande partie due à l'utilisation de modèles animaux. Cependant, la grande diversité de la sensibilité gustative et la spécificité des études de mammifères justifie dans les tissus humains. Nous décrivons une technique de biopsie pour collecter les cellules gustatives de vie des papilles sur la langue humaine.
Tags
TechniqueCollectFungiform PapillaeHuman TongueSense Of TasteCriticalQuality Of FoodPotentially IngestedStimulate Metabolic ProcessesAlimentary CanalDigestionProgressUnderstandingBiochemical EventsMolecular EventsTaste TransductionAnimal ModelsReceptor SpecificityReceptor DensityBiopsy TechniqueRounded Pink Anterior StructuresDiameterTaste BudsIsolation Of Viable Taste Bud CellsIn Situ HybridizationImmunohistochemistryMolecular Biology TechniquesIdentification Of Taste-specific Novel Proteins
