Encyclopedia of Experiments: Immunology
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Transcript
For myeloid cell depletion, intraperitoneally deliver 100 milligrams per kilogram of cyclophosphamide, and orally deliver 125 milligrams per kilogram of disulfiram to 6 to 8-week-old NOD/SCID female mice once a day for two days. 24 hours after the second dose of cyclophosphamide and disulfiram, resuspend 5 x 106 freshly isolated human PBMC and 2.5 x 106 human tumor cell line cells in 200 microliters of PBS plus 50% matrigel per animal, and inject the entire volume subcutaneously into the right flank of each experimental animal. Measure and record the primary tumor volume 2 times a week for 4 to 6 weeks.