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维持细胞培养基的Wolbachia

JoVE 신문
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JoVE 신문 생물학

Maintaining Wolbachia in Cell-free Medium

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维持细胞培养基的Wolbachia

DOI:

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13:33 min

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July 04, 2007

•

Courtney Gamston, Jason Rasgon

¹Malaria Research Institute, Bloomberg School of Public Health,Johns Hopkins University

Chapters

00:06Introduction
01:24Lysing Infected Anopheles Cells
03:34Filtering Lysates and Isolation of Wolbacchia
09:31Assay of Wolbacchia Viability Using Live/Dead Stain
12:11Conclusion
13:22End Credits

Summary

자동 번역

本视频介绍了净化按蚊细胞系的Wolbachia pipientis，然后在无细胞培养基培养的共生细菌的方法。细菌的生存能力的检测证明。

Tags

Wolbachia Cell-free Medium Video Protocol Purify Symbionts Cultured Mosquito Cells Fluorescent Assay Viability Extracellular Phase Replicate Eukaryotic Cells Micro-injection DNA Extraction

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