JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
An Assay To Evaluate the Anticancer Effects of Lactobacillus Cell-Free Supernatant
내레이션 대본
For LCFS treatment, serially dilute the prepared thawed LCFS stock solution in fresh growth medium to 25%, 12.5, and 6% concentrations and use a 200-microliter pipette to carefully remove as much supernatant as possible from each well of the plated colorectal cancer cell line cultures.
Then gently add 200 microliters of the LCFS-supplemented growth medium in triplicate to each set of cancer cells, and return the plate to the incubator for an additional 24 to 48 hours.
