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Chapters
- 00:04Title
- 00:45Introduction
- 01:33Preparing Geltrex-coated Culture Dishes
- 02:59Adapting Human iPSCs
- 05:20Passaging Human iPSCs
- 07:36Representative Results
- 08:07Conclusion
O protocolo a seguir fornece instruções para a adaptação humana tronco pluripotentes induzidas (iPS) células para alimentação livre de cultura usando completa KnockOut Feeder livre de substituição de soro médio (KSR-FF). Uma vez adaptado, instruções para a manutenção contínua também são fornecidos.
Tags
Feeder-Free AdaptationCulturePassagingHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-Free MediumReprogrammedIPS CellsEmbryonic Stem CellsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Fibroblast Feeder LayersHuman Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated HiPS CellsUndefined Conditions
