JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:02Introduction
- 01:24Preparing Spinal Cord Emulsion
- 08:43Loading Glass Syringes with Emulsion
- 12:37Injecting Rats with Spinal Cord Emulsion
- 15:51Monitoring Clinical Signs (CS)
- 17:31CS 0.0 – No Symptoms of EAE
- 18:50CS 0.5 – Distal Limp Tail
- 19:29CS 1.0 – Limp Tail
- 20:21CS 2.0 – Weakened Hindlimbs
- 21:59CS 3.0 – Moderate Hindlimb Paralysis
- 23:06CS 3.5 – Paralysis of Single Hindlimb
- 23:28CS 4.0 – Complete Hindlimb Paralysis
- 23:44CS 5.0 – Complete Hindlimb Paralysis and Incontinence
- 24:07Caring for Animals with EAE
- 24:53Conclusion
- 26:39End Credits
Cette vidéo montre le score d'induction et cliniques d'un modèle animal de sclérose en plaques: chronique récurrente encéphalomyélite allergique expérimentale chez des rats DA. La maladie, provoquée par les rats immunisant avec une émulsion contenant la moelle épinière de rats et de toute l'adjuvant complet de Freund, présente des signes cliniques ressemblant à la maladie humaine.
Tags
InductionClinical ScoringChronic-Relapsing Experimental Autoimmune EncephalomyelitisMultiple SclerosisChronic Inflammatory DiseaseCentral Nervous SystemDemyelinationGlial ScaringBrainSpinal CordDemyelinated PlaquesParaparesisParalysisBlindnessIncontinenceExperimental Autoimmune Encephalomyelitis (EAE)MS ModelVaccinationRabiesViruses Grown On Rabbit Spinal CordsCNS DiseaseNon-human Primates Immunized With A CNS HomogenateSpecies/strainImmunizing AntigenLewis RatsMyelin Basic Protein In Emulsion With AdjuvantGuinea PigsDA RatsDA Rat Spinal CordComplete Freund’s AdjuvantAscending Flaccid Paralysis
