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Chapters
- 00:05Title
- 01:47Transfer Fosmid to SW016 Bacteria
- 04:49Insertion of the galK Gene by Recombineering
- 08:48Replacement of galK with Tag Sequences by Recombineering
- 10:48Representative Results
- 11:34Conclusion
La capacidad de producir transgenes de<em> Caenorhabditis elegans</em> Utilizando ADN genómico realizado por fosmids es particularmente atractivo ya que todos los elementos de regulación nativos se mantienen. Descrito es un procedimiento sencillo y robusto para la producción de transgenes a través de recombinería con la<em> GALK</em> Marcador de selección.
Tags
C. ElegansTransgenesRecombineeringGalK Selectable MarkerGFP Fusion ProteinsGene RegulationExpression PatternTandem Affinity Purification (TAP)PromoterReporter GeneCDNA3′ Untranslated RegionAlternative PromotersWorm Genomic DNAFosmid DNA ClonesE. Coli Based Recombineering ProcedureGFP InsertionTAP-tag InsertionPositive SelectionNegative Selection
