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Isolatie en In vitro Activering van Caenorhabditis elegans Sperma
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Isolation and In vitro Activation of Caenorhabditis elegans Sperm
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Isolatie en In vitro Activering van Caenorhabditis elegans Sperma

DOI:
10.3791/2336-v

•

05:46 min

•

January 31, 2011

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, , ,
1Waksman Institute of Microbiology,Rutgers University

Chapters

  • 00:05Title
  • 00:21Subscription
  • 00:40Introduction
  • 01:29Enrichment of Male Population, Identification, and Isolation of Males
  • 02:29Dissection of Males and in vitro Activation
  • 04:25Visualization of Spermtids and Spermatozoa
  • 04:54Images of C. elegans Spermatid Isolation and Activation
  • 05:21Conclusion

Summary

자동 번역

자동 번역

Een protocol voor het isoleren en het activeren van spermatiden van man<em> C. elegans</em> Wordt hier beschreven. Het snijden van de achterste eind van de mannelijke releases spermatiden. De spermatiden kan worden geactiveerd door toevoeging van protease.

Tags

Caenorhabditis ElegansIsolationIn Vitro ActivationSpermMalesHermaphroditesGametogenesisSpermathecaOocytesX Chromosome Non-disjunctionHim PhenotypeTail MorphologyMating StructuresDissectionStereo MicroscopeSpermatids

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