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Chapters
- 00:02Title
- 00:07Introduction
- 00:37Materials and Equipment
- 01:09Preparing Embryoid Body Cryo-Blocks
- 05:14Cutting
- 06:37Conclusion
Pluripotenten Stammzellen in Suspension wachsende Differenzierung in Embryoidkörpern (EBS). Hier zeigen wir, wie eine hohe Qualität EB Kryoschnitten nützlich für die Untersuchung zellulärer und molekularer Aspekte der Embryogenese zu erhalten, unter Wahrung ihrer Organisation als Aggregate.
Tags
Pluripotent Stem CellsCryosectionsEmbryoid BodiesES CellsDifferentiationAggregatesEctodermMesodermEndodermImmunofluorescenceIn Situ HybridizationTarget ProteinsMRNACryosection TechniqueAnalytical ProceduresHistology Staining AnalysisHematoxilin And EosinDAPIOct4NestinEmbryogenesisTri-dimensional Spherical Structure
