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Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
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Chapters
- 00:00Title
- 01:12Purifying and Labeling Prion Rods
- 03:37Prion Inoculation and Cell Recovery
- 06:51Cell Staining and Flow Cytometry
- 08:09Cellular Trafficking Response to Purified Prion Rods
- 08:58Conclusion
Here we describe a novel assay for monitoring prion uptake and trafficking by immune cells immediately following intraperitoneal inoculation by purifying and fluorescently labeling aggregated prion rods from infected brain material then monitoring their uptake and movement from the injection site and characterizing the cells mediating these events.
Tags
Immune CellsTraffickingFluorescent Prion RodsIntraperitoneal InfectionAbnormal FormHost-encoded Prion Protein (PrPC)Protease ResistantPathologicInfectiousPrion DiseasesChronic Wasting Disease (CWD)ScrapiePrion HypothesisImmune SystemPeripheral Prion PathogenesisComplement SystemDendritic CellsMacrophagesPrion UptakeTraffickingInoculation Site
