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Chapters
- 01:34Introduction
- 02:10Generation of GFP-tagged Sla1 and Sla1 Clatherin-binding Mutant Yeast Strains
- 03:22Fluorescence Microscopy
- 05:09Fluorescence Image Analysis
- 06:41Preparation of Yeast Cytosolic Extract
- 09:54GST-fusion Protein Affinity Assay
- 11:53Preparation of Total Cell Extract
- 13:36Co-immunoprecipitation Assay
- 15:16Representative Results
- 16:39Conclusion
Clathrin-vermittelte Endozytose hängt Adapter Proteine, die Fracht Auswahl und Clathrin-Mantel Montage zu koordinieren. Hier beschreiben wir Verfahren, die Adapter-Clathrin physikalische Interaktion und Live Cell Imaging Ansätze benutzt als Modell die Hefe endocytischen Adapter-Protein Sla1p studieren.
