JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 01:34Introduction
- 02:10Generation of GFP-tagged Sla1 and Sla1 Clatherin-binding Mutant Yeast Strains
- 03:22Fluorescence Microscopy
- 05:09Fluorescence Image Analysis
- 06:41Preparation of Yeast Cytosolic Extract
- 09:54GST-fusion Protein Affinity Assay
- 11:53Preparation of Total Cell Extract
- 13:36Co-immunoprecipitation Assay
- 15:16Representative Results
- 16:39Conclusion
