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Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
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JoVE 신문 신경과학
Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
DOI:

11:41 min

November 14, 2010

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Chapters

  • 00:00Title
  • 00:20Introduction
  • 01:20Preparations for Automated Sholl Analysis
  • 02:14Tracing Neurons in NeuronJ
  • 04:52Using Bonfire to Build Preliminary .swc Files from NeuronJ Data
  • 06:27Using NeuronStudio to Finalize .swc Files
  • 07:57Using ‘bonfire’ to Extract Morphological Data and ‘bonfire_results’ to View the Data
  • 09:06Using ‘bonfire_export’ to Export Data into Excel
  • 11:21Conclusion

Summary

자동 번역

We have developed a computer program to analyze neuronal morphology. In combination with two existing open source analysis tools, our program performs Sholl analysis and determines the number of neurites, branch points, and neurite tips. The analyses are performed so that local changes in neurite morphology can be observed.

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