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免疫印迹检测样品制备
JoVE 신문
생물학
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JoVE 신문 생물학
Western Blotting: Sample Preparation to Detection
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免疫印迹检测样品制备

DOI:
10.3791/2359-v

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07:45 min

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October 14, 2010

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,
1Research and Development,EMD Chemicals Inc.

Chapters

  • 00:00Title
  • 00:58Sample Preparation
  • 01:52SDS-PAGE
  • 03:21Protein Transfer
  • 04:55Membrane Blocking and Detection
  • 06:56Representative Western Blotting Results
  • 07:26Conclusion

Summary

자동 번역

자동 번역

Western印迹分析技术，用于检测特定的蛋白质，在一个给定的样本，组织匀浆或提取。

Tags

Western BlottingSample PreparationDetectionProteinsExtractGel ElectrophoresisNative ProteinsDenatured ProteinsPolypeptide Length3-D StructureMembrane TransferNitrocellulosePVDFAntibody Detection

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