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Rapid PCR Thermocycling using Microscale Thermal Convection
JoVE 신문
생물학
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JoVE 신문 생물학
Rapid PCR Thermocycling using Microscale Thermal Convection
DOI:

09:02 min

March 05, 2011

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Chapters

  • 00:05Title
  • 01:21Basic Design of the Convective Thermocycler
  • 02:48Preparation and Loading of the PCR Reaction Mixture
  • 03:42Running the Reaction in the Convective Thermocycler
  • 04:25Performing Gel Electrophoresis Analysis
  • 05:31Representative Results for Rapid PCR Thermocycling Using Microscale Thermal Convection
  • 08:33Conclusion

Summary

자동 번역

We describe a novel method to perform DNA replication via the polymerase chain reaction (PCR). Thermal convection is harnessed to continuously shuttle reagents between denaturing, annealing, and extension conditions by maintaining opposing surfaces of the reactor at constant temperature. This inherently simple design promises to make rapid PCR more accessible.

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