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Chapters
- 00:05Title
- 01:01Introduction
- 01:33Preparation of Solutions
- 02:29Isolation of Retina from Mouse Eye
- 04:08Treatment of Retina with Enzyme MIxture and Mounting in Recording Chamber
- 06:10Localization of EGFP Expressing Ganglion Cells and Light Stimulation
- 09:51Analysis of Anatomy of Recorded Ganglion Cells
- 11:21Response of Retinal Ganglion Cell to Light Stimulation and Correlation with Morphology
- 12:45Conclusion
Este artículo proporciona una descripción de cómo analizar y record de la preparación aislada de la retina en el ratón. En particular, se describe la forma de registrar las respuestas de la luz de una población de células ganglionares marcados con fluorescencia y, posteriormente, identificar y analizar su morfología.
Tags
Isolated Retinal PreparationLight ResponseGenetically Labeled Retinal Ganglion CellsVertebrate VisionRod And Cone PhotoreceptorsON And OFF PathwaysBipolar Cells (BCs)Retinal Ganglion Cells (RGCs)Inner Plexiform Layer (IPL)Excitatory InputIlluminationParallel SignalingBrain Visual NucleiFluorescent Reporter Mouse LinesRGC SubtypesElectrophysiological Recording
