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Ex vivo Uitbreiding van Tumor-reactieve T-cellen door middel van Bryostatin 1/Ionomycin en de Gemeenschappelijke Gamma Chain Cytokines Formulering
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
DOI:

07:20 min

January 14, 2011

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Chapters

  • 00:05Title
  • 00:55Introduction
  • 01:48Mouse Model of Breast Cancer
  • 02:14Isolation of Lymphocytes
  • 03:44Determine Fold Expansion of T Cells by Cell Counts and Flow Cytometry Analysis
  • 04:58Determine Tumor-Specificity of the ex vivo Expanded T Cells
  • 05:47Selective Expansion of Tumor-Reactive T Cells with Effector Anti-Tumor Function
  • 06:53Conclusion

Summary

자동 번역

Een efficiënte protocol voor de<em> Ex vivo</em> Uitbreiding van de tumor-reactieve T-cellen van tumor-drainerende lymfeklieren of andere secundaire lymfoïde weefsels van tumor-dragende hosts wordt beschreven. Dit protocol breidt selectief tumor-specifieke T-cellen voor gebruik in de adoptieve immunotherapie van borstkanker.

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