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Ensayo in vitro de metilación del ADN tRNA con Entamoeba histolytica y Dnmt2 tRNA metiltransferasa (Ehmeth) Enzima
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Chapters
- 00:00Title
- 01:43tRNA Preparation
- 05:28Expression and Purification of the Recombinant E. histolytica Dnmt2 (Ehmeth) Protein in E. coli BL21
- 08:06 in vitro tRNA Methylation Assay
- 10:30Representative Results
- 12:02Conclusion
Este protocolo describe la preparación de un sustrato sintético para el tRNA<em> Entamoeba histolytica</em> DNA / tRNA metiltransferasa 2 (Dnmt2) Ehmeth homólogo y la medida de su actividad metiltransferasa. Este enfoque experimental puede ser utilizado para investigar la actividad de otras proteínas Dnmt2.
Tags
In Vitro TRNA Methylation AssayEntamoeba HistolyticaDNA Methyltransferase Dnmt2Ehmeth EnzymeProtozoan ParasitesAmebiasisInfectious AgentsClinical Manifestations5-methylcytosine (5mC)Single DNA MethyltransferaseControl Of Repetitive ElementsDual DNA/tRNAAsp Methyltransferase ActivityFunctional SignificanceTRNA Methyltransferase ActivityDnmt2 Proteins
