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Chapters
- 00:05Title
- 01:46Liver Decellularization
- 04:28Recellularization of Decellularized Liver Matrix
- 06:14In Vitro Culture of the Recellularized Liver Graft
- 08:04Representative Results
- 09:07Conclusion
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
Tags
DecellularizationRecellularizationWhole LiversLiver MicrostructureMicrofabrication TechniquesSynthetic BiomaterialsScaffoldsCell Binding MotifsBlood VesselsSkinCell AttachmentReorganizationPerfusion-decellularizationLiver BioscaffoldMajor Blood VesselsRecellularize BioscaffoldsAdult Primary HepatocytesLiver GraftFunctional In VitroTransplantation In Vivo
