JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:49Preparation of Coverslips or “Video” Dishes
- 03:40Preparation of Neuronal Cultures and Media
- 04:53Permeabilization of Neuronal Cultures and Incorporation of Rhodamine-actin onto F-actin Barbed Ends
- 06:13Variations to this Procedure
- 07:35Representative Actin Labeling Results
- 08:47Conclusion
Tags
F-actinBarbed EndsRhodamine-actinPermeabilizedNeuronal Growth ConesAxonsMolecular Guidance CuesGrowth Cone CytoskeletonActin Filament MeshworkActin PolymerizationDynamic RemodelingGuidance CuesActin MonomerPolymerization NucleiChick RetinalDorsal Root Ganglion (DRG)Actin Depolymerizing Factor (ADF/cofilin)
