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Chapters
- 00:05Title
- 00:50Introduction
- 02:52Microfluidic Device Fabrication
- 05:45Formation of Uniform Tumor Spheroids using the Hanging Drop Method
- 07:04Introduction of Spheroids into the Microfluidic Device
- 09:18Introduction of Apoptosis-detecting Agent into the Microfluidic Device
- 10:50Introduction of Therapeutic Agents into the Microfluidic Device
- 11:59Time Lapse Microscopy and Estimation of Drug Diffusivity Coefficients
- 14:25Representative Results
- 15:30Concluding Remarks
Se presenta el procedimiento para la fabricación y el funcionamiento de un dispositivo de microfluidos que recrea heterogéneos microambientes tumorales<em> In vitro</em>. La variabilidad en la apoptosis en el tejido tumoral se cuantificó utilizando tinciones fluorescentes y el coeficiente de difusión eficaz del fármaco quimioterapéutico doxorrubicina en el tejido tumoral se evaluó.
Tags
Microfluidic DeviceTumor MicroenvironmentIn VitroDrug DeliverySystemic ClearanceHeterogeneousVasculatureNutrientsViable CellsQuiescent CellsNecrotic CellsCancer DrugsPenetrationMonolayersIn Vitro ModelPDMSSoft LithographyMulticellular Tumor SpheroidsHanging Drop MethodRectangular ChambersContinuous Medium PerfusionLinear GradientsFluorescent StainsApoptosis VariabilityChemotherapeutic Drug DoxorubicinDiffusion CoefficientCell Lines
