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A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
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JoVE 신문 생물학
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
DOI:

10:21 min

February 01, 2011

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Chapters

  • 00:05Title
  • 01:05Replication of the Metagenomic Library Plates
  • 03:39Measuring the Growth of E. coli Clones.
  • 06:35Addition of the Assay Mix to Each Plate
  • 07:47Reading Absorbance of Assayed Clones
  • 09:17Representative Results of a Solution-Based Screen for a Cellulose Activity from Metagenomic Libraries
  • 09:56Conclusion

Summary

자동 번역

This protocol describes a high throughput screen for cellulolytic activity from a metagenomic library expressed in Escherichia coli. The screen is solution based and highly automated, and uses one-pot chemistry in 384 well microplates with the final readout as an absorbance measurement.

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