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Chapters
- 00:05Title
- 01:08Introduction
- 02:00The Creation of Virtual Slices of Immunohistochemically Stained Images
- 04:15Computer-Assisted Two-Dimensional Analysis
- 05:42Three-Dimensional Reconstruction of Two Dimensional Immunohistochemically Stained Virtual Slice Images
- 07:25Islet Mapping: Collecting Image Stacks
- 10:21Islet Mapping: Mapping Image Stacks
- 12:36Representative Results for the Large-Scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture
- 15:47Conclusion
Novel computer-assisted methods of large-scale procurement and analysis of immunohistochemically stained pancreatic specimens are described: (1) Virtual Slice capture of the entire section; (2) Mass analysis of large-scale data; (3) Reconstruction of 2D Virtual Slices; (4) 3D islet mapping; and (5) Mathematical analysis.
Tags
Computer-assistedLarge-scale VisualizationQuantificationPancreatic IsletIslet MassSize DistributionArchitectureEndocrine CellsBeta-cellsAlpha-cellsDelta-cellsPancreas WeightTotal Beta-cell MassIslet Size DistributionFunctional LimitLarge Animal PancreataIslet PlasticityCellular CompositionArchitectureSpecies Variation
