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RNA Interference in Ticks
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
RNA Interference in Ticks

RNA Interference in Ticks

DOI:
10.3791/2474-v

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09:06 min

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January 20, 2011

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, ,
1Department of Veterinary Pathobiology, Center for Veterinary Health Sciences,Oklahoma State University, 2(CSIC-UCLM-JCCM),Instituto de Investigación en Recursos Cinegéticos IREC

Chapters

  • 00:00Title
  • 01:45Generation of dsRNA
  • 02:28Preparation and Injection of Ticks
  • 04:50Treatment of Ticks after Injection
  • 06:31Tick Analysis after RNAi
  • 07:34Representative Tick RNAi Results
  • 08:30Conclusion

Summary

자동 번역

자동 번역

A method for RNA interference (RNAi) by injection of dsRNA into unfed ticks is described. RNAi is the most widely used gene-silencing technique in ticks where the use of other methods of genetic manipulation has been limited.

Tags

RNA InterferenceTicksHematophagous EctoparasitesVectors Of Human DiseasesCattle IndustryControl Tick InfestationsRNAiGene Expression DisruptionSmall Interfering RNAs (siRNAs)Double-stranded RNA (dsRNA)Post-transcriptional Gene SilencingFunctional Genomics StudiesGenetic Manipulation

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