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Chapters
- 00:04Title
- 00:22Introduction
- 00:53Preparation of Dissection Solution
- 02:15Adult Drosophila Selection
- 02:32Sex Differentiation
- 03:04Decapitation and Removal of Brain
- 04:19Removal of Trachea and Enzyme Treatment
- 05:27Stabilizing Brain in Recording Chamber
- 07:58Conclusion
Cette vidéo montre la procédure pour isoler cerveaux entiers de l'adulte de drosophile en préparation pour l'enregistrement à partir de neurones unique en utilisant la technologie standard de cellules entières. Il comporte des images de cellules GFP étiqueté et les neurones considérés lors de l'enregistrement.
Tags
Whole Cell RecordingsBrainAdult DrosophilaProcedureSingle NeuronsDissecting SolutionCaptureOptic LobesTracheaIsolated BrainNeuronsHandlingDamage PreventionHolderRecording ChamberElectrophysiology SetupGAL4 LineGFP ExpressionProjection Neurons (PNs)Live BrainNomarski ImageTargeted NeuronSpontaneous ActivityAction PotentialsSynaptic InputIn Situ Preparation
