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Isolation of Mouse Salivary Gland Stem Cells
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Isolation of Mouse Salivary Gland Stem Cells

Isolation of Mouse Salivary Gland Stem Cells

DOI:
10.3791/2484-v

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09:52 min

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February 08, 2011

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, , , ,
1Department of Cell Biology,University Medical Center Groningen, University of Groningen, 2Department of Radiation Oncology,University Medical Center Groningen, University of Groningen

Chapters

  • 00:05Title
  • 01:32Mechanical and Enzymatic Salivary Gland Tissue Digestion
  • 05:00Preparation of Salivary Gland Tissue for Plating
  • 07:32Plating Salivary Gland Cells
  • 08:39Examination of Salispheres and Proliferating Cells Using Phase-Contrast Microscopy and Immunostaining
  • 09:34Conclusion

Summary

자동 번역

자동 번역

An optimized protocol for the isolation of stem cells from the mouse salivary gland is described. The method employs enzymatic and mechanical digestion, and permits isolation of salispheres containing cells with characteristics of stem cells.

Tags

IsolationMouse Salivary Gland Stem CellsSalivary GlandsCell TypesProductionExcretionSalivaAcinar CellsProtein ProductionMucous ProductionDuctal CellsEpithelial-type MorphologySaliva CompositionDiseasesSjögren’s SyndromeRadiotherapy TreatmentXerostomiaDry MouthDental CariesRegenerative CapacityStem Cell-like Populations

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