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An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
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Chapters
- 00:00Title
- 02:17Fluorescent Labeling of Exopolysaccharides and Bacterial Cells in Biofilms
- 04:12Biofilm Image Acquisition and Data Analysis
- 04:58Biofilm Structural Analysis and Quantification
- 06:25Transcriptome Analysis of Biofilms
- 09:51Differential Gene Expression in S. mutans Biofilms Formed in the Presence of Different Dietary Carbohydrates
- 10:28Conclusion
Biofilms formed on tooth surfaces are highly complex and exposed to constant innate and exogenous environmental challenges, which modulate their architecture, physiology and transcriptome. We developed a toolbox to examine the composition, structural organization and gene expression of oral biofilms, which can be adapted to other areas of biofilm research.
Tags
Analytical Tool-boxComprehensive Biochemical EvaluationStructural EvaluationTranscriptome EvaluationOral BiofilmsMutans StreptococciMicrobial CellsExtracellular MatrixBiofilm DevelopmentCell ClustersMicrocoloniesExtracellular EnzymesSucrose SubstrateDental BiofilmsExopolysaccharides (EPS)Caries DiseaseGlucosyltransferasesTooth SurfacesEnvironmental ChallengesDiet-host-microbial InteractionsOral CavityMatrix CompositionPhysiology Profile
