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小鼠呼吸道上皮细胞和实验香烟烟雾暴露在空气分离液界面
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JoVE 신문 의학
Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
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小鼠呼吸道上皮细胞和实验香烟烟雾暴露在空气分离液界面

DOI:
10.3791/2513-v

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10:47 min

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February 21, 2011

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, ,
1Department of Medicine, Pulmonary and Critical Care Medicine, Brigham and Women’s Hospital,Harvard Medical School, 2Cellular and Molecular Pathology, School of Medicine,University of Pittsburgh

Chapters

  • 00:00Title
  • 01:15Dissection of Mouse Tracheas
  • 03:40Isolation of Mouse Tracheobronchial Epithelial Cells (MTEC)
  • 08:25Application of Cigarette Smoke to Cultured Epithelial Cells
  • 09:44Representative Results of Healthy Mouse Respiratory Epithelial Cell Cultures
  • 10:28Conclusion

Summary

자동 번역

자동 번역

从小鼠的呼吸道和肺上皮细胞可以分离，气液界面的差异化的呼吸道上皮模式培养。协议是分离，培养，这些细胞暴露卷烟主流烟雾，为了研究这种环境毒素的分子反应。

Tags

Mouse Respiratory Epithelial CellsIsolationExperimental Cigarette SmokeAir-liquid Interface (ALI)Differentiated Respiratory EpitheliumMainstream Cigarette Smoke (CS) ExposureAirway Epithelial CellsTracheaFully Differentiated Epithelial CellsCalibrated Mainstream CS DeliveryALI Culture SystemRespiratory EpitheliaPhysiological SettingMolecular ResponsesLung Cellular ResponsesEnvironmental Air PollutionHuman HealthChronic Obstructive Pulmonary Disease (COPD)Tobacco-related Diseases

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