This content is Free Access.
Chapters
- 00:00Title
- 00:50Introduction
- 01:50Lipid Extraction
- 02:49Thin Layer Chromatography (TLC)
- 06:21Fatty Acyl Methylester (FAME) Reaction
- 08:29Gas-Liquid Chromatography (GLC)
- 10:21Representative Results for Polar Glycerolipid Profiling by TLC Coupled with GLC
- 12:21Conclusion
Composition of polar lipid extracts and the fatty acid composition of individual glycerolipids are determined in a simple and robust lipid profiling experiment. For this purpose, glycerolipids are isolated by thin layer chromatography and subjected to transmethylation of their acyl groups. Fatty acyl methylesters are quantified by gas-liquid chromatography.
Tags
Arabidopsis ThalianaPolar Glycerolipid ProfilingThin Layer Chromatography (TLC)Gas-liquid Chromatography (GLC)Biological MembranesGlycerolipidsPhosphatidylcholinePhosphatidylethanolamineBilayer MembranesChemical ExchangeChloroplastGlycolipidsMonogalactosyldiacylglycerol (MGDG)Digalactosyldiacylglycerol (DGDG)Sulfoquinovosyldiacylglycerol (SQDG)PhotosynthesisCrystal StructuresPhotosystem I And IIPhosphate StarvationExtraplastidic MembranesPhospholipidsBiosynthesisGenetic StudiesBiochemical Studies
