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Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

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면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

DOI:

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10:44 min

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February 02, 2011

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Srinivas S. Somanchi, Vladimir V. Senyukov, Cecele J. Denman, Dean A. Lee

¹Division of Pediatrics,MD Anderson Cancer Center – University of Texas

Chapters

00:05Title
01:13Introduction
01:40Isolation of Cell Fractions from Buffy Coat
03:34NK Cell Expansion
06:34NK Cell Cytotoxicity Assay
08:33Representative Results
09:55Conclusion

Summary

자동 번역

Here we describe a method to efficiently expand and purify large numbers of human NK cells and assess their function.

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