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Chapters
- 00:05Title
- 01:15Kidney Dissection and Capsule Removal
- 03:23Enzymatic Digestion of Kidneys and Isolation of Nephrogenic Zone Cells
- 06:30Phase Contrast and Fluorescent Microscopy of Purified Nephrogenic Zone Cells
- 07:24Conclusion
In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.
Tags
IsolationCultureCellsNephrogenic ZoneEmbryonic Mouse KidneyEpithelial-mesenchymal InteractionOrganogenesisCongenital Kidney DiseaseEmbryonic Stem CellsRegenerative MedicineGenetic StudiesPathwaysCatalog Of Gene TranscriptionOrgan CultureCandidate ProteinsSiRNAMorpholinosSignalingStem/progenitor Cell DifferentiationRenewal
