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波谱与自发拉曼显微荧光背景的抑制

JoVE 신문
생체공학

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JoVE 신문 생체공학

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

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波谱与自发拉曼显微荧光背景的抑制

DOI:

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15:04 min

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May 18, 2011

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Zachary J. Smith*¹, Florian Knorr*¹, Cynthia V. Pagba, Sebastian Wachsmann-Hogiu²

¹Center for Biophotonics Science and Technology,University of California, Davis, ²Department of Pathology and Laboratory Medicine,University of California, Davis

Chapters

00:05Title
06:26Preparation of the Pump Beam (Kerr Beam)
07:46Setting Up the Kerr Gate and Collection System to Maximize the Collected Time-gated Signal
11:14Collecting a Time-gated Spectrum
12:31Representative Time-gated Raman Spectra
14:01Conclusion

Summary

자동 번역

我们讨论一个复杂的非线性光学系统，采用超快全光开关，隔离荧光信号的拉曼的建设和运营。使用这个系统，我们能够成功地分离的拉曼光谱和荧光信号，利用脉冲能量和平均的权力，保持生物安全。

Tags

Fluorescence Background Raman Spectroscopy Biological Samples Ultrafast Pulses Picosecond Timescale Raman Scattered Light Fluorescence Signals Nonlinear Optical System Optical Kerr Gate Laser Power Repetition Rate Nonlinear Medium All-optical Shutter Peak Efficiency Pulse Energies Average Powers Biologically Safe Optical Power

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