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Chapters
- 00:05Title
- 01:08Preparation of Zebrafish Embryos
- 02:00Preparation for Electroporation
- 03:23Electroporation
- 05:28Mounting and Time-lapse Live Imaging
- 07:19Conclusion
The present video demonstrates a method which takes advantage of the combination of electroporation and confocal microscopy to perform live imaging on individual neural progenitor cells in the developing zebrafish forebrain. In vivo analysis of the development of forebrain neural progenitor cells at a clonal level can be achieved in this way.
Tags
Time-lapse Live ImagingClonally Related Neural Progenitor CellsDeveloping Zebrafish ForebrainBrain DevelopmentIn Vivo EnvironmentElectroporationFluorescent MarkersGenetic LabelingLow Melting Point AgaroseGlass Bottom Culture DishesConfocal MicroscopeWater Dipping Objective LensCentral Nervous SystemZebrafish Embryo
